Analytical techniques for characterization of raw materials in cell culture media
نویسندگان
چکیده
Raw materials are a critical part of any cell culture medium; therefore, it is of utmost importance to understand and characterize them for high-quality product. The raw material characterization (RMC) program at SAFC focuses on individual screening of raw materials both analytically and biologically. The goal of the program is to develop the best-in-class knowledge base of the raw materials used in SAFC's media formulations and their impact on performance of products.
منابع مشابه
P-63: Comparison of The Efficacy of Two Culture Media (Handmade vs. Commercial) on In Vitro Development of Vitrified and Non-Vitrified Mouse Embryos
Background: While assisted reproductive techniques (ARTs) are considered to be safe, many concerns exist about the safety of these techniques. Two important and indispensable parts of these techniques are in vitro culture and vitrification. While much progress has been made in developing culture conditions, it remains suboptimal. In order to investigate the effect of culture medium on in vitro ...
متن کاملDevelopment of protein-free medium for therapeutic protein production in mammalian cells: recent advances and perspectives
Mammalian cell culture media used for the manufacture of therapeutic protein have advanced systematically from serum-containing into animal-free, protein-free and chemically defined formulations over the past decades. Initially driven by patient safety concerns associated with the use of animal-derived medium components, and later by inconsistent cell culture performance due to variability in p...
متن کاملCharacterization of Raw Material Influence on Mammalian Cell Culture Performance: Chemometrics Based Data Fusion Approach
Near-infrared, Raman, fluorescence and X-ray fluorescence spectra of multiple soy hydrolysate lots manufactured by different vendors were analyzed for comprehensive characterization of raw materials used in mammalian cell culture processes. The variability of soy hydrolysates, as well as the correlation between multiple spectra and cell culture performance was addressed. The identified composit...
متن کاملSwitching industrial production processes from complex to defined media: method development and case study using the example of Penicillium chrysogenum
BACKGROUND Filamentous fungi are versatile cell factories and widely used for the production of antibiotics, organic acids, enzymes and other industrially relevant compounds at large scale. As a fact, industrial production processes employing filamentous fungi are commonly based on complex raw materials. However, considerable lot-to-lot variability of complex media ingredients not only demands ...
متن کاملEstimating the effective density of engineered nanomaterials for in vitro dosimetry
The need for accurate in vitro dosimetry remains a major obstacle to the development of cost-effective toxicological screening methods for engineered nanomaterials. An important key to accurate in vitro dosimetry is the characterization of sedimentation and diffusion rates of nanoparticles suspended in culture media, which largely depend upon the effective density and diameter of formed agglome...
متن کامل